Journal: Foods

Article Title: Screening of a Fraction with Higher Amyloid β Aggregation Inhibitory Activity from a Library Containing 210 Mushroom Extracts Using a Microliter-Scale High-Throughput Screening System with Quantum Dot Imaging

doi: 10.3390/foods13233740

Figure Lengend Snippet: Inhibitory effect of the EtOAc extract of t037 on extracellular Aβ deposition in SH-SY5Y cells. ( A ) SH-SY5Y cells were co-cultured with 25 μM Aβ and 25 nM QDAβ and different concentrations of f5 and f5f3. Images were collected using an inverted fluorescence microscope. 1.2% DMSO was used as the negative control (without Aβ). DIA: bright field image; QD: fluorescence image of QD and Aβ aggregation; Merge: merged image. ( B ) The QD field’s mean gray value of each group was determined. Data are represented as mean ± SD ( n = 3). (**: p < 0.01, ***: p < 0.001, Welch’s t -test).

Article Snippet: The images of each well were observed by an inverted fluorescence microscope (ECLIPSE Ti-E, Nikon, Tokyo, Japan) equipped with a color CMOS camera (DS-Ri2, Nikon).

Techniques: Cell Culture, Fluorescence, Microscopy, Negative Control